FastQCFastQC Report
Wed 18 Oct 2017
HMTWKBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTWKBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338599935
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17108540.5052729853595512No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT10410680.30746255163929664No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5662200.16722389506660715No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG5250220.15505673384136945No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG3882410.11466068355860731No Hit
TGCCAGCTCTGACCGAAATCTTTGGAGATGATTCTGTATTGCAATTTGGT3642240.10756765207293971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAT2251250.020.7511776
GTTATAC3123600.020.7139783
TATACCG3192600.020.4869775
TTATACC3187350.020.1335544
ATACCGG3214450.020.1188226
TACCGGT3190200.019.9373517
GGGGTAT3125150.019.32654422
GGTATTC3159950.019.17693724
TGTTATA3522550.019.1226332
GGGGGTA3253600.018.9854621
TGGACCA2653100.018.9707555
GGGTATT3202100.018.93825523
TCAGGGG3300250.018.8913518
GGCTTCA3334300.018.68144214
TGGCATA3142200.018.50635136
TTCAGGG3398800.018.36677617
TGGCTTC3433350.018.04899613
ACCGGTG3618600.017.8352958
GCTTCAG3547000.017.76205415
TTAGTCC695850.017.6854298
Produced by FastQC (version 0.11.2)