FastQCFastQC Report
Wed 18 Oct 2017
HMTWKBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTWKBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338599935
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCTT7316300.013.9107661
CCTACAA4932950.013.8868431
TACACGT5108500.013.8699142
TACTCCA5857450.013.8688463
TACACCT4736200.013.8600421
CTACTCC5868600.013.8560682
CCACACT4377150.013.8434362
ACACCTT4737900.013.8427752
CTTCAGC5545450.013.840821
AAGCCTT4541200.013.8306781
TCCTTAC4714400.013.8214544
CACCTTC4739050.013.8177523
AATCCTT4727850.013.8174192
CCTCTCT2727900.013.81376654
CTCCAGT5873050.013.8074665
CCTATAG4093700.013.806972
CCTTACG4713850.013.8043785
TCCACAC4376950.013.7959681
CCTTCGT4738850.013.7946215
AGGACAC4942650.013.7945425
Produced by FastQC (version 0.11.2)