FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163149824
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT20972761.2854908136462349TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT16907251.0363020679691324TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT14619450.8960751315306353TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT14223910.8718311580893889TruSeq Adapter, Index 8 (97% over 36bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT8262240.506420405332463Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG3130860.19190091188820407Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2112060.12945524231763805TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCGAAATCTCGTATG1937230.11873932514937925TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)