FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29205076
Sequences flagged as poor quality0
Sequence length101
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT2215947275.87541289055369TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG1738520.5952800807640425TruSeq Adapter, Index 6 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA705550.2415847163006869TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGAAT557170.1907784797409875TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTCCGCGAAATCTCGTATG481390.1648309355538058Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA435140.1489946473688341TruSeq Adapter, Index 6 (97% over 36bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA348790.11942786931970319TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA316810.10847771805147845TruSeq Adapter, Index 6 (96% over 32bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG294380.10079754628955596TruSeq Adapter, Index 3 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)