FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45537525
Sequences flagged as poor quality0
Sequence length101
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT3426558175.24691120125655TruSeq Adapter, Index 6 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA1336660.29352934749967197TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG1177630.2586065009022778TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA943320.20715223324060758TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGAAT858180.18845556494341756TruSeq Adapter, Index 6 (97% over 36bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA778790.17102159153357588TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTCCGCGAAATCTCGTATG726320.15949922618763318Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA548840.120524775995182TruSeq Adapter, Index 6 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)