FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26553446
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1335470750.293686928619366TruSeq Adapter, Index 6 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA584360.22006936500821778TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG512800.19311994382951275TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGAAT331650.12489904323529231TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA288550.1086676282995435TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTCCGCGAAATCTCGTATG273910.10315421960675085Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)