FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30982568
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1284230441.45009542139954TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG1510840.4876419540174978TruSeq Adapter, Index 6 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA1104470.35648110253481896TruSeq Adapter, Index 6 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA331230.10690850416272789TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGAAT323000.10425217173734597TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)