FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2679280
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT155944558.20388313278194TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG60140.22446328864471052TruSeq Adapter, Index 6 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA45190.16866471589382223TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGAAT39070.14582275835299036TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTCCGCGAAATCTCGTATG33320.12436176883341794Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG27920.10420710041503688TruSeq Adapter, Index 3 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)