FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29975857
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1668442555.659542944843906TruSeq Adapter, Index 6 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA767230.255949312808638TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG544790.18174292731647337TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGAAT414000.13811114724759996TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTCCGCGAAATCTCGTATG343770.11468229248624985Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA308060.10276937203163199TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)