FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31793667
Sequences flagged as poor quality0
Sequence length101
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT2161886767.99740023697171TruSeq Adapter, Index 6 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA761600.23954456087119488TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG630670.1983634036300374TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGAAT539730.1697602230029018TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTCCGCGAAATCTCGTATG451030.14186158520185796Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA443150.13938310418864236TruSeq Adapter, Index 6 (97% over 36bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA360330.11333389130608937TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA345860.1087826704607556TruSeq Adapter, Index 6 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)