FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27483052
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1207924043.9515960600009TruSeq Adapter, Index 7 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA416870.15168257149897327TruSeq Adapter, Index 2 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG391520.14245870509578049TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGAAT306180.11140684084140291TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAT282300.10271784953141304TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCGGCTATGATCTCGTATG275900.10038914164263851Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)