FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26843213
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1098884740.93715234461687TruSeq Adapter, Index 3 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA359220.13382153619240736TruSeq Adapter, Index 10 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG330000.12293610306635051TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGAAT285650.10641423588152432TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)