FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27676196
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1766939663.84329696176454TruSeq Adapter, Index 3 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA619240.22374462155131436TruSeq Adapter, Index 10 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG539610.19497260389397444TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGAAT452560.1635195819541096TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG315660.1140546916201923TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA299260.10812902177741478TruSeq Adapter, Index 3 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTAATGCGCATCTCGTATG285760.10325118379707963Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)