FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43634727
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2546108858.35051517567648TruSeq Adapter, Index 3 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA974450.22331983422286564TruSeq Adapter, Index 10 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG726280.16644540940980335TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGAAT655260.15016938229039453TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG584740.13400794280207137TruSeq Adapter, Index 3 (97% over 36bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG453190.10385993706343115TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)