FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26438053
Sequences flagged as poor quality0
Sequence length101
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2075199178.49288674926251TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGAAT534100.20201941496977863TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG444410.16809482907080942TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA385970.14599032689737024TruSeq Adapter, Index 3 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTAATGCGCATCTCGTATG337990.12784224314854048Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA330320.1249411217989464TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAAGCGCATCTCGTAT272220.10296522213644099TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)