FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24775388
Sequences flagged as poor quality0
Sequence length101
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2148801386.73128751808045TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGAAT557650.22508224694604176TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG446510.1802232118423332TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA386720.15609039099609662TruSeq Adapter, Index 3 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTAATGCGCATCTCGTATG348360.14060728332488678Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA324980.13117049872236108TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG258600.10437778007755115TruSeq Adapter, Index 3 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)