FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21714024
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1127429551.92172118811327TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGAAT289490.13331937000714378TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG243760.11225924775619665TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA224040.10317755935058376TruSeq Adapter, Index 3 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)