FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29744713
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1633239154.908551311286814TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGAAT415370.1396449849759855TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAAATCTCGTAT366130.12309078255352472TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG329500.11077598899676726TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACAGCGATAGATCTCGTATG315980.10623064340879672Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT297890.10014889032548405TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)