FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27730245
Sequences flagged as poor quality0
Sequence length101
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT2414521087.07175143962846TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATNTCGTAT796710.2873072343933492TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGAAT617860.2228108695036773TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAAATCTCGTAT535830.19322945037088565TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG470990.1698470388559495TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT469850.16943593538391025TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACAGCGATAGATCTCGTATG459300.1656314251821432Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGAAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT413830.14923416652106752TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA413050.14895288519809327TruSeq Adapter, Index 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAATAGATCTCGTAT408550.1473301083347803TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGATAGATCTCGTAT369270.13316506940346182TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAACACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT362790.13082827072029116TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAATCACAGCGATAGATCTCGTAT342360.1234608637608503TruSeq Adapter, Index 13 (97% over 35bp)
GATCAGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT324180.11690484523306592TruSeq Adapter, Index 13 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA304780.10990887386678337TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTAAACTCCAGTCACAGCGATAGATCTCGTAT287060.10351873919613765TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACATCTGAACTCCAGTCACAGCGATAGATCTCGTAT286940.1034754651464493TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)