FastQCFastQC Report
Tue 6 Dec 2022
HMTJCDRX2_n01_EN001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMTJCDRX2_n01_EN001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35526931
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT2274882564.0326207743641TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGAAT579710.16317480392550654TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG437490.12314320085796322TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT406730.11448498042231679TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA400450.11271730732947352TruSeq Adapter, Index 8 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)