FastQCFastQC Report
Wed 26 Sep 2018
HMT73BGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT73BGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63667774
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9661891.517547951338773No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN743180.11672781272359231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACA115700.032.2003614
TACAGGC102900.031.57272717
ATTACAG117050.031.444715
TTACAGG111900.031.16368916
CTGGGAT127150.030.64938710
TTGAACC45550.030.4964132
ACAGGCG50900.030.30150618
TACTCGG22700.029.939563
GGACTAC34200.029.25603113
AGGCCGA34950.029.20979111
AGGCTGA114150.029.154511
CGCTTGA23950.029.04527329
CTACTCG23100.029.033162
AATCGCT19100.028.86168926
GGGACTA36850.028.74070512
AGTAGCT101550.028.673345
GTAGCTG103200.028.5170826
GGAGAAT59900.028.48869322
GCTACTC53000.028.074111
AAGTGCT68450.028.041395
Produced by FastQC (version 0.11.5)