FastQCFastQC Report
Wed 26 Sep 2018
HMT73BGX7_n02_HAP1_ATAC_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT73BGX7_n02_HAP1_ATAC_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166736561
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1803620.10817183640965222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCT683350.034.2037126
GAATCGC673750.033.9734425
AGAATCG702700.033.56104324
GGGATTA4789450.033.52151512
GGATTAC4500550.033.4797613
GATTACA4573100.033.3994814
ATTACAG4583700.032.90115
ATCGCTT821800.032.58776527
TGGGATT5075950.032.41591611
TACAGGC4036500.032.3079117
CGCTTGA804800.032.23846429
TTACAGG4465150.032.19550316
TCGCTTG813650.031.94707528
TTGAACC1836450.031.43887332
ACAGGCG1905800.031.22803518
CTGGGAT5189500.030.9664210
AGGCCGA1358550.030.95902611
CGGATCA738000.030.43172824
GCGGATC740250.030.20182423
CTACTCG911350.029.9843732
Produced by FastQC (version 0.11.5)