FastQCFastQC Report
Wed 26 Sep 2018
HMT73BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT73BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63667774
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8486911.332999328671362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACA139900.033.0734814
ATTACAG139750.032.9742415
TACAGGC122250.032.94875317
AATCGCT19450.032.47439226
TTACAGG135850.032.4138916
CTGGGAT157750.031.15537810
ACAGGCG58450.030.87851118
GGGACTA47800.030.6203712
GGACTAC45050.030.44327213
TTGAACC53400.030.22103732
CGCTTGA25700.029.64922529
AGTAGCT125500.029.4531975
GGAGAAT67700.029.11801722
AGGCTGA128800.029.11691711
GTAGCTG127400.028.9082136
GACTACA47450.028.80842814
AGGCCGA39650.028.46595211
AAGTGCT88200.028.3982125
CTACTCG27050.028.3427752
GCTACTC60900.028.25421
Produced by FastQC (version 0.11.5)