FastQCFastQC Report
Wed 26 Sep 2018
HMT73BGX7_n01_HAP1_ATAC_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT73BGX7_n01_HAP1_ATAC_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166211516
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC694150.034.52783225
AATCGCT706400.034.51189826
AGAATCG730050.033.73280324
GGATTAC4725000.033.6044613
GGGATTA5037950.033.59253312
GATTACA4790550.033.542414
ATCGCTT841900.033.2887327
ATTACAG4791250.033.05705615
CGCTTGA830200.032.99360329
TGGGATT5320850.032.59119411
TCGCTTG847300.032.36767628
TACAGGC4256950.032.35996617
TTACAGG4685700.032.28925716
TTGAACC1886400.031.79556732
ACAGGCG1985800.031.49882118
CTGGGAT5432200.031.31234610
CGGATCA752850.030.87349924
AGGCCGA1411000.030.85750611
GCGGATC755000.030.35054623
GGACTAC1545000.030.18768313