FastQCFastQC Report
Tue 9 Oct 2018
HMT72BGX7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT72BGX7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522099955
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57034641.0924084450457383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA2498550.085.722536
GCTCGAT8155900.081.151357
CTCGATG8190650.080.822228
CGCTCGA1682550.080.780446
CGGCTCG575300.078.2053455
AGCAGTG5733550.077.4882662
GGGCTCG955000.076.861865
CCGCTCG389250.072.2496955
AGGCTCG594800.072.240165
GCAGTGG6480750.068.543643
GAGCTCG552950.067.984735
TCGCTCG536600.067.031385
TGGCTCG800900.066.698375
CAGTGGT6823900.065.120224
AAGCAGT7288150.061.269571
AGCTCGA2277700.058.6928526
TGCTCGA3300350.054.954816
CGCGCTC159550.054.0396464
TCGGCTC213950.053.993184
AGTGGTA8701500.051.3532685
Produced by FastQC (version 0.11.5)