FastQCFastQC Report
Tue 9 Oct 2018
HMT72BGX7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT72BGX7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522099955
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC10889578820.857268221752673No Hit
AGCCAATT7193538513.778086803321024No Hit
GCTGTCCA6762396212.95230182504038No Hit
CTAGGTGA6714178712.859948819570382RNA PCR Primer, Index 38 (100% over 8bp)
AAACCGAG5798608511.106318712477192No Hit
CGCTATGT5362564410.271145110518157No Hit
TTGAGATC5221993510.001903754234187No Hit
GGGGGGGG188522443.610849573813888No Hit
TCGTTCAG42038640.8051837506862072No Hit
TCCGGAGA36537820.6998242319327531No Hit
ATTACTCG35754350.6848181015453257No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)