FastQCFastQC Report
Tue 9 Oct 2018
HMT72BGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT72BGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522099955
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA1620900.017.9958081
ACTGATG1544700.017.9330081
ACGAGGA1372050.017.9030881
ACAGCCG1849050.017.8860741
CCTCTGA2216400.017.864321
CTGATCC1721400.017.8579221
GTGCAGC2509950.017.8111691
TTGAGAG1027450.017.6758985
TGCCAAA1862500.017.5945031
TTGAACG1455150.017.5872971
AAGACCT2051600.017.5193311
TCTGACA1543850.017.4437873
CGTGAGC1565950.017.3985751
GGGACCT1042000.017.3527931
CTGCGGA1149300.017.3136621
CACCGAA1042950.017.2502528
ATGCGAT1552150.017.2359091
TGCTACC1768800.017.2232041
ACCCACT1771800.017.2112311
TGGGAAC1584700.017.17572610
Produced by FastQC (version 0.11.5)