FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20504293
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2928891.428427695605013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2735021.3338767642463947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG229340.11184974775770129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA242400.068.2074953
GTTAAAA286500.062.49783756
GGGGGTT301300.058.25226652
CCGGGGA33400.055.32819749
AAAGGGG687500.052.0049668
AAGGGGG701400.051.5717269
AAAAGGG697600.051.20585367
AGGGGGG740500.050.43824470
GGGTTAA337700.049.7861754
TTGGGGG580450.049.49897439
TTTGGGG588650.049.0513538
AAAAAGG740000.048.68890866
TTTTGGG587600.047.97657437
TTTTTGG606250.046.18970536
TATGGGG16150.046.1651138
TGGGGGG676800.045.49799340
GGGCCGG68750.044.2400646
GGCCGGG66700.043.86919447
GGGGGAA827850.043.0881821
GGGTTTA22200.042.879854
Produced by FastQC (version 0.11.5)