FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359116
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1979175.891937045341692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG478431.4242735291070627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG130770.3892988512453872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG83950.24991694243366408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG72230.21502681062517637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG49400.14706250096751644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA156000.068.57696554
GGGGTTA154850.068.341553
GGTTAAA160050.067.37546555
GGGGGTT169050.066.0767552
GTTAAAA171600.064.3731756
TTGGGGG297900.064.040139
TTTGGGG297950.063.5219838
TGGGGGG319200.061.42236340
GGGGGGT199850.059.78328751
TTTTGGG311850.059.7711237
AGGGGGG364900.058.5411370
GGCCGGG31850.057.14170547
GGGGCCG32800.057.08215345
GGGCCGG32650.056.70128646
GCCGGGG32000.056.43636348
TTTTTGG342650.054.3290436
AAGGGGG396150.053.66773269
AAAGGGG398950.053.1673968
CCGGGGA22300.052.25884649
AAAAGGG407800.052.0666267