FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291311
Sequences flagged as poor quality0
Sequence length76
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG14408149.45951234247934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3302411.33633814033799No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC87042.9878720679960593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG82172.820696781103357No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG73372.518614127169932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG60162.065146870526688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG34821.1952861374956663No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG20000.6865514862123298No Hit
GGGGGGGGGGGCCCCCGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC17070.5859716934822234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG14400.49431707007287745No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGGG12440.4270350244240691No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG10760.36936469958223345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTATTTGGGGGGGGGG10220.35082780945450054No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG9960.3419026401337402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTGGGGGGGGGG9340.320619544061158No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC9210.31615695940077787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG8550.293500760355771No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC7500.25745680732962367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTATTTTTGGGGGGGGGG7090.2433825018622709No Hit
GGGGGGGAAAAAAGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC6840.2348006082846168No Hit
GGGGGGGGGGGCCCCCCGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC6520.2238157845052195No Hit
GGGGGGGGGGGGGGGGGGGTGGGGGAAAAAATTTTTTTTTGGGGGGGGGG6110.20974147903786677No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG5810.1994432067446818No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG5150.17678700769967493No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG4580.15722029034262353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGCC4570.15687701459951736No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG4060.13936995170110295No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC3770.12941495515102416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAATATTTTTTTTTGGGGGGGGGG3630.12460909474753785No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC3620.12426581900443169No Hit
GGGGGGGGGGGGGGCGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG3090.10607220461980496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG500.070.0382235
TAAATGG301.01774276E-770.0382236
TATATGG1200.070.0382236
ATTAGGG500.070.0382237
TTATTTG2900.070.0382235
TATAAGG252.8264694E-670.0382236
TTATTGG1500.070.0382236
TTCTGGG150.002218417870.0382237
GGTTTTG150.002218417870.0382235
TTATATG700.070.0382235
ATAATTG207.8972786E-570.0382235
TTATAGG207.8972786E-570.0382236
TCTCGGT500.070.0382236
ATTAAGG150.002218417870.0382236
GATCTCG500.070.0382234
TTAAGGG207.8972786E-570.0382237
TATTTGG3350.070.0382236
ATTGAGG150.002218417870.0382238
CTCGGTG500.070.0382237
TAATATG150.002218417870.0382235
Produced by FastQC (version 0.11.5)