FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186419
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1931268.832982150264886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG452082.0676732135972107No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG132550.6062424448378834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC109280.4998127074453707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG99360.4544417149686314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG76070.34792050380096406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG44510.20357488660682146No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG26060.11919032902659554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA154150.069.093454
GGGGTTA152500.068.9458653
GGTTAAA156000.068.6168555
GGGGGTT165250.066.9258552
GTTAAAA166450.066.3068156
TTTGGGG285000.065.6894938
TTGGGGG288650.065.46361539
TTTTGGG296300.062.23930737
TGGGGGG318350.061.5330940
GGGGGGT196300.060.60091451
GGGGCCG30800.060.22867645
GCCGGGG29350.059.1509448
GGGCCGG30550.058.88574246
CCGGGGA20250.058.76545349
AGGGGGG362550.058.66750770
GGCCGGG29750.058.5909447
TTTTTGG317700.057.92175736
TATGGGG11450.055.0218538
AAGGGGG391800.054.0784469
AAAGGGG391600.054.0407468