FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317946
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1537586.63337282231769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG385491.6630672155434165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC96770.4174816842152492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG87110.3758068565876858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG79700.3438388987491512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG66060.2849936970058837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG34210.14758756243674356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA122550.068.3170254
GGGGTTA120850.068.170153
GGTTAAA123850.067.8616155
GGGGGTT130200.066.3643552
GTTAAAA132950.065.4532756
TTTGGGG232400.064.7171938
TTGGGGG234150.064.612439
TTTTGGG240150.061.27319737
TGGGGGG258800.060.78412640
GGGGCCG26900.059.32878545
GGGGGGT158150.059.0184751
AGGGGGG291400.058.0911570
GCCGGGG26700.056.7656848
TTTTTGG260550.056.42814336
GGGCCGG27800.056.27380446
GGCCGGG27100.055.9241847
CCGGGGA17950.053.8154449
AAGGGGG316550.053.24122669
AAAGGGG316750.053.11157668
AAAAGGG324250.051.90244367