FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3394535
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2324616.848095541804695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG562261.6563682507324273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG156030.4596505854262808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC133890.39442810281820634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG123270.36314252172978034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG95390.2810105065936866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG51710.1523330883316861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA190650.068.6755354
GGGGTTA189200.068.403453
GGTTAAA193450.068.0674755
GGGGGTT206700.065.8938752
GTTAAAA206950.065.5553156
TTTGGGG346900.065.0190738
TTGGGGG350700.064.71612539
TTTTGGG360750.061.12478337
TGGGGGG386350.060.8922940
GGGGGGT242650.060.08477851
GGGGCCG39800.059.53636645
GGGCCGG40300.058.01348546
AGGGGGG448600.057.78484770
GGCCGGG40400.056.83114647
TTTTTGG389700.056.6083936
CCGGGGA28500.056.1173149
GCCGGGG40400.055.9664748
AAGGGGG483950.053.3903969
AAAGGGG483700.053.37127368
AAAAGGG494650.052.15210767