FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270964
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1907595.831889314587381No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501141.53208656530613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG132830.4060882357616898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG67180.20538287795279922No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG61150.18694794562092398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG54870.16774871261193947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA148100.067.7039154
GGGGTTA147200.067.6175353
GGTTAAA149550.067.4525455
GGGGGTT163000.064.8196152
GTTAAAA160700.064.0139256
TTTGGGG281400.063.13195838
TTGGGGG283800.062.9499939
TTTTGGG295450.059.3113137
AGGGGGG347150.058.79178270
TGGGGGG310950.058.6580940
GGGGGGT196150.057.700951
GGGGCCG31600.056.2687645
GGGCCGG32400.054.5528246
AAGGGGG376800.053.81852369
AAAGGGG378650.053.30900668
TTTTTGG329750.053.12228836
GGCCGGG32900.052.76548447
AAAAGGG387300.051.96473767
GCCGGGG33550.050.91175548
AAAAAGG407850.049.48452466