FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798000
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2299108.216940671908505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG596852.1331308077198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG163610.5847390993566833No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC145580.5203002144388849No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG107590.38452466047176553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG66660.23824160114367404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG51210.18302358827734097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG46820.16733380986418872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA187850.068.99377454
GGGGTTA186650.068.8197753
GGTTAAA189500.068.7501155
GGGGGTT204750.067.122152
GTTAAAA199300.066.334356
TTTGGGG344100.063.48232338
TTGGGGG350450.063.05109439
TTTTGGG355600.060.494137
AGGGGGG427150.059.73017570
TGGGGGG381200.059.46975740
GGGGGGT246200.059.16618751
TTTTTGG379050.056.64912836
GGGCCGG35100.056.2362146
GGGGCCG35450.056.07789645
GCCGGGG34550.055.72025748
GGCCGGG34800.055.5191447
CCGGGGA22650.054.3899349
AAGGGGG467200.054.2033969
AAAGGGG468150.054.0123768
GGTTTAA17450.053.958955