FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180162
Sequences flagged as poor quality0
Sequence length76
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG9515752.81746428214606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2185612.131304048578501No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG64883.601203361419167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC58023.220434941885636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG33591.8644331213019394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG32091.7811747205293014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG25361.4076220290627324No Hit
GGGGGGGGGGGCCCCCGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC11400.6327638458720485No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG10660.5916897014908804No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG7990.4434897481155849No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGGG5850.324707763013288No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG5350.29695496275574207No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG5230.290294290693931No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTGGGGGGGGGG5010.2780830585806108No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC4740.2630965464415359No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG4550.2525504823436685No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTATTTGGGGGGGGGG4260.23645385819429182No Hit
GGGGGGGAAAAAAGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC4150.2303482421376317No Hit
GGGGGGGGGGGCCCCCCGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC3920.21758195401916053No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC3740.20759094592644398No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG3580.1987100498440293No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG3450.19149432177706732No Hit
GGGGGGGGGGGGGGGGGGGTGGGGGAAAAAATTTTTTTTTGGGGGGGGGG3110.17262241760193603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTATTTTTGGGGGGGGGG2770.15375051342680476No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG2540.1409842253083336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGCC2460.13654377726712627No Hit
GGTTGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC2420.13432355324652256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGAAAATTTTTTTTTGGGGGGGGGG2290.12710782517956062No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC2100.11656176108169314No Hit
GGGGGGGGGGGGGGCGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2040.11323142505078763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAATATTTTTTTTTGGGGGGGGGG2030.1126763690456367No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG2020.1121213130404858No Hit
GGGGGGGGGGGCCCCCGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2000.11101120103018394No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1940.10768086499927843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTG150.00222062170.00973535
TTTTGCG150.00222062170.00973537
TATATGG455.456968E-1270.00973536
ATTAGGG150.00222062170.00973537
TTATTTG1400.070.00973535
TATAAGG150.00222062170.00973536
TGTTTTG150.00222062170.00973535
TTATTGG455.456968E-1270.00973536
TTTTTGC150.00222062170.00973536
TGTTTGG150.00222062170.00973536
TTATATG353.6852725E-970.00973535
TTTTATG1200.070.00973535
TTATAAG150.00222062170.00973535
ATAAAGG150.00222062170.00973536
TAATTTA150.00222062170.00973533
TTAAGGG150.00222062170.00973537
ATATGGG600.070.00973537
AATATGG150.00222062170.00973536
TTAATTG150.00222062170.00973535
AATGTTT150.00222062170.00973530
Produced by FastQC (version 0.11.5)