FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124364
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG21241718.892191496703916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG491044.367268962720257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG140911.2532418327160955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC128281.1409116620596178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG82760.7360605640166351No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG74910.6662433162214372No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG57340.5099771959970258No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG25380.2257276113429459No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG19120.1700516914451192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGGG13960.12415908015553682No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG13440.11953424335891223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTGGGGGGGGGG12580.1118854748106485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG12390.11019563059649722No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG11930.10610442881486778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA160700.069.600353
GGGTTAA162600.069.5618454
GGTTAAA164650.069.27579555
GGGGGTT173100.068.5568652
GTTAAAA170150.068.4146756
TTTGGGG294900.066.6517138
TTGGGGG298550.066.1064739
TTTTGGG296400.065.3961237
CCGGGGA21200.064.2174949
TTTTTGG304750.063.40054336
TGGGGGG325000.062.36330440
GGCCGGG32000.062.3452247
GGGCCGG32600.062.1585146
GGGGGGT203800.061.9531351
GCCGGGG32000.061.58231748
GGGGCCG33250.061.47513645
GGGTTTA11800.061.39154454
GGGCCCC7300.061.360379
CGGGGAA22800.060.32499350
TATGGGG7150.060.21925738
Produced by FastQC (version 0.11.5)