FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390833
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1741077.282273584144104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424911.7772466751128164No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG121160.5067689796819769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG60180.25171143279350755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG58440.24443363463696544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG53890.22540261072186976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA136600.068.74370653
GGGTTAA138200.068.5809654
GGTTAAA140250.068.130455
GGGGGTT148550.066.64377652
GTTAAAA148900.065.27869456
TTGGGGG257550.064.4016839
TTTGGGG257000.064.2044238
TTTTGGG266100.061.0879937
TGGGGGG279150.060.73493640
GGGGGGT176150.059.54050451
AGGGGGG320250.058.87856370
GGGGCCG28150.057.1905445
GGGCCGG28350.056.7858946
TTTTTGG289500.056.0126136
GGCCGGG28000.055.74934847
GCCGGGG28300.053.92276448
AAGGGGG347350.053.91986569
AAAGGGG346800.053.8484168
CCGGGGA19400.053.03728549
AAAAGGG356150.052.42490467