FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3488.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3488.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248877
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1709965.26323403440635No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433211.333414592180621No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG117950.36304852415157607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG64040.19711426440582394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG51780.15937814820320992No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG44950.13835549945411907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA138350.068.146154
GGGGTTA137250.067.87736553
GGTTAAA141300.066.98035455
GGGGGTT151900.065.1995452
GTTAAAA152700.063.30832356
TTTGGGG260700.062.73525238
TTGGGGG263350.062.3440339
TGGGGGG280350.059.48833540
AGGGGGG313250.059.2960870
GGGGGGT177400.059.1811751
TTTTGGG280550.057.65923337
GGGCCGG29900.054.66320846
GGGGCCG29800.054.61258745
GCCGGGG29850.054.05627448
AAGGGGG342700.053.9511469
GGCCGGG30150.053.51428247
AAAGGGG345100.053.42873868
TTTTTGG313000.051.6367636
AAAAGGG356450.051.63669267
CCGGGGA19200.050.85405749