FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037959
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1538067.547060564025086No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388461.9061227433917953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG106350.5218456308492958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG49920.24495095338031825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG47610.23361608354240687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG47340.2322912286262874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA113250.068.9748854
GGGGTTA112550.068.5953953
GGTTAAA114800.068.3940655
GGGGGTT125050.065.9613552
GTTAAAA122800.065.4492356
TTTGGGG221600.064.38817638
TTGGGGG223750.064.1636339
TTTTGGG231750.060.85671637
GGGGCCG24500.060.72010445
TGGGGGG243850.060.2669840
AGGGGGG274250.058.89711470
GGGGGGT150000.058.60598851
GGCCGGG24550.058.59456347
GCCGGGG24700.058.3861648
GGGCCGG25400.057.59828646
CCGGGGA16150.055.6909849
TTTTTGG254900.055.28036536
AAGGGGG296400.054.0810169
AAAGGGG296750.053.8350968
AAAAGGG301200.053.00224767
Produced by FastQC (version 0.11.5)