FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926098
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1280924.377570402631764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316351.0811326209853531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG88040.30087850782851433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC74890.2559381128041508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG64640.22090852732888647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG50620.17299488943979321No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG29340.10027005247261028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA101050.067.7166854
GGTTAAA102800.067.0486455
GGGGTTA101150.066.8884653
GGGGGTT111800.064.2702652
TTGGGGG195750.063.93575739
TTTGGGG199400.062.59954538
GTTAAAA113550.062.5804656
TGGGGGG214850.059.88088640
GGGGGGT132800.057.90019651
AGGGGGG242700.057.5100270
TTTTGGG213050.057.25506637
GGGGCCG21600.054.1178845
GGGCCGG21800.053.2993946
AAGGGGG264200.052.5508269
AAAGGGG267150.052.0553968
GCCGGGG21850.051.90492648
TTTTTGG237800.051.1998136
GGCCGGG22200.051.08135647
AAAAGGG275700.050.32676367
GGGGGCC24450.048.09594744