FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835142
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2754799.716585624282665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG656052.3139934437146357No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG194510.6860679288726985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG95680.3374786871345421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG91200.3216770094760686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG79950.28199645732030354No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG29170.1028872627896592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA213350.069.0700954
GGTTAAA215200.068.85519455
GGGGTTA212400.068.82371553
GGGGGTT231650.066.9406552
GTTAAAA226450.066.8265756
TTTGGGG400250.065.35988638
TTGGGGG404050.065.1471939
TTTTGGG409800.062.99858537
TGGGGGG440000.061.35247440
GGGGGGT274100.060.44425651
GGGCCGG40800.059.4493746
GGGGCCG41350.059.33578545
GCCGGGG39950.059.22905348
TTTTTGG434850.059.21132736
AGGGGGG505500.059.0978870
GGCCGGG40250.058.8714447
CCGGGGA27000.058.06976349
GGCCCCC10450.054.9251210
AAGGGGG549200.054.10036569
AAAGGGG549450.054.01235668