FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971542
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1962186.603238318691104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG459641.5468063382580493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG135640.45646334462040244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG81670.2748404700320574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG69410.2335824295937934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG51460.1731760816438065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA153750.068.54294653
GGGTTAA155200.068.53284554
GGTTAAA156300.068.3439355
GGGGGTT168700.066.4465452
TTGGGGG289400.064.79281639
GTTAAAA169050.064.7824656
TTTGGGG288650.064.5421838
TGGGGGG312450.061.58027340
TTTTGGG302000.060.73858637
GGGGGGT200450.059.92402651
AGGGGGG361650.058.5142270
GGCCGGG31450.057.53758647
GGGCCGG31900.057.05124746
GGGGCCG32400.057.03595445
GCCGGGG31900.056.06949248
TTTTTGG330800.055.4739136
CCGGGGA21100.054.9032449
AAGGGGG394700.053.5081569
AAAGGGG395750.053.2654468
AAAAGGG404700.052.09435767