FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653839
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG824383.106367793976952No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209050.7877267611185155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG57210.2155744941573321No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC48100.1812468653901009No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG36080.13595398967307362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG29990.11300610172659306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA65700.066.4770854
GGGGTTA66000.065.5916453
GGTTAAA67550.065.1819255
GGGGGTT74250.062.4030852
TTGGGGG129150.061.2223739
TTTGGGG131050.060.1801838
GTTAAAA75100.060.12264356
TGGGGGG140050.058.38076840
AGGGGGG156300.057.6385870
GGGGGGT88100.056.7594351
TTTTGGG145450.053.18829337
AAGGGGG170950.052.24670469
AAAGGGG174050.051.08448468
GGGCCGG15850.049.4604346
AAAAGGG183050.048.63018867
GGCCGGG16050.047.75563447
GGGGCCG16450.047.657345
GCCGGGG16300.046.1643148
AAAAAGG198500.044.77626866
TTTTTGG172850.044.72908836
Produced by FastQC (version 0.11.5)