FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020458
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1656368.197943238612236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG396221.961040516556147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG111820.5534388737603058No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC99450.49221513142069767No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG67170.3324493753396507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG61760.30567326814019397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG39420.19510427833689192No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA125150.068.7772254
GGGGTTA124900.068.4681753
GGTTAAA126600.068.3455455
GGGGGTT135600.066.7278452
GTTAAAA135850.065.62430656
TTTGGGG235050.065.4476538
TTGGGGG238650.065.0307739
TTTTGGG242400.062.21978837
TGGGGGG259450.061.35520640
GGGGGGT159200.060.1750451
GGGCCGG27100.059.40744846
GGCCGGG26650.059.362947
GGGGCCG27400.058.7584645
CCGGGGA18750.058.61557849
AGGGGGG297100.058.57656570
GCCGGGG26750.058.36026448
TTTTTGG262800.057.23776236
AAGGGGG323200.053.5931469
AAAGGGG323300.053.56553668
GGTTTAA9400.052.87567555