FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128948
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1787508.396165617948395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434412.0404913600520067No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG123670.5808972318722674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC111560.5240146776717891No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG61040.2867143772417175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG59780.28079596119773714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG54330.2551964632297266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA138150.068.7504454
GGGGTTA137500.068.4359353
GGTTAAA139700.068.2994955
GTTAAAA147450.066.0864656
GGGGGTT150650.066.08440452
TTTGGGG260400.064.1457638
TTGGGGG261700.064.127239
TTTTGGG271100.060.8507937
TGGGGGG283650.060.2629340
AGGGGGG318500.059.49584670
GGGGGGT177500.059.44085751
GGGCCGG28800.059.17840246
GGGGCCG29300.059.1296345
GCCGGGG28650.057.90297748
GGCCGGG28950.057.6642747
TTTTTGG295400.055.86618436
CCGGGGA19350.055.1588249
AAGGGGG344550.054.661169
AAAGGGG345950.054.45385768
AAAAGGG353550.053.25359767