FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449365
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1219474.978718974101451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305471.2471395647443317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG86430.3528669675609801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC68120.27811289864924177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG66310.27072322826528505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG49640.20266477229812624No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG29250.11941870648106755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA95200.068.04568554
GGGGTTA95000.067.6363553
GGTTAAA97350.066.9451755
GGGGGTT104550.064.9689752
TTGGGGG185450.063.43588339
TTTGGGG184300.063.20626438
GTTAAAA106900.062.7011456
TGGGGGG203050.060.0921440
GGGGGGT126150.058.4821551
TTTTGGG195000.058.46237637
AGGGGGG237800.056.91570770
GGGGCCG21500.055.99752845
GGGCCGG21650.055.44676646
GGCCGGG22100.053.53033447
AAGGGGG253500.053.22502569
AAAGGGG253750.053.10468768
GCCGGGG21850.053.02910248
AAAAGGG261300.051.4609967
TTTTTGG221750.051.43313236
CCGGGGA15200.049.046949