FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523595
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1764096.99038474874138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG422451.6740007806323915No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG119560.4737685722154308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC107930.4276835229107681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG71470.28320709147069956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG62200.24647378046001836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG46050.18247777476179816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA137250.068.4431654
GGGGTTA135950.068.322653
GGTTAAA139550.067.8892755
GGGGGTT148000.066.3529252
GTTAAAA149600.064.9427356
TTTGGGG259650.064.35847538
TTGGGGG261750.064.2006539
TGGGGGG284100.060.62725440
TTTTGGG273700.060.0304137
GGGGGGT177050.059.6750351
AGGGGGG325750.058.51194470
GGCCGGG29350.058.4223447
GGGGCCG30400.058.12997445
GGGCCGG30400.057.8974646
GCCGGGG30100.056.27355248
TTTTTGG298700.054.9589536
CCGGGGA20150.054.18823249
AAGGGGG349650.054.06305369
AAAGGGG350650.053.81546868
AAAAGGG357650.052.81321367