FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671952
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG18536111.08650248332488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG484562.89816932543518No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG125460.7503803936955128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG81580.48793266792348106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG52170.31203048891355734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG39340.23529383618668478No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG34850.20843899824875356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA142400.069.0869554
GGGGTTA141750.068.786553
GGTTAAA144700.068.4606755
GTTAAAA150800.066.7571556
GGGGGTT156700.066.625852
TTTGGGG268900.063.77153838
TTGGGGG272400.063.4052539
TTTTGGG272850.061.98322737
TGGGGGG294700.059.88995440
AGGGGGG328950.059.8287970
GGGGGGT183800.059.73119451
GGCCGGG27800.059.17663647
TTTTTGG286000.058.90948536
GGGCCGG28650.058.883546
CCGGGGA17750.057.37726249
GCCGGGG29000.056.7296548
GGGGCCG29900.056.6576345
GGGTTTA13200.055.41375454
GGTTTAA13350.054.01435555
AAGGGGG363350.053.824169