FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3467.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3467.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360170
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1449056.139600113551142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365461.548447781303889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG95650.4052674171775762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC89840.3806505463589487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG52300.2215942071969392No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG49620.21023909294669452No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG39380.16685238775172975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA117050.068.4763854
GGTTAAA118350.068.0538655
GGGGTTA118000.067.423753
GTTAAAA126550.065.0520756
GGGGGTT130100.064.7015252
TTTGGGG216900.063.71751838
TTGGGGG219550.063.19818539
TGGGGGG236200.060.20909540
TTTTGGG229450.059.5001137
GGGGGGT150500.059.20546351
AGGGGGG268250.058.9039870
GGGGCCG23500.057.19263545
GGGCCGG23550.056.6253446
GGCCGGG24400.054.3658447
GCCGGGG24250.053.983948
AAGGGGG292100.053.81769669
AAAGGGG293150.053.45890468
TTTTTGG256100.053.2174336
AAAAGGG300150.052.15494567
CCGGGGA15250.050.4917449